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Seurat vignette practice. 2,700 Human Peripheral Blood Mononuclear Cells (PBMC) sequenced on the Illumina NextSeq 500. |
Testing various QC metrics and their influence on clustering results |
April 2024 |
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Bulk RNA sequencing data analysis of human airway smooth muscle cells and their response to Dexamethasone. Analysis includes differential gene expression, functional pathway and gene enrichment. |
Exploring all model designs and contrasts |
April 2024 |
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Bulk RNA sequencing data analysis of 3 innate immune cells from the blood and/or BALF of healthy, smoker and COPD patients. Analysis includes, differential gene expression, functional pathway and gene enrichment. |
Practicing pathway enrichment analysis |
March 2024 |
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40 color spectral flow cytometry data analysis of 5 mouse lymphoid tissues. Steps will include QC, transformation, visualization and classification of immune cells based on their fluorescence-tagged protein markers. |
Exploring automated transformation methods |
Sept 2023 |